Program works in Internet Explorer 5 but not Netscape 4.

Place "x" or leave blank if the position can be occupied by any amino acid.

-3
-2
-1
Phospho-residue
+1
+2
+3
Following changes you might make in the table above

Consensus Sequence

Optimum Sequence

Step 1.

To find proteins that have the potential phosphorylation sites, copy the consensus sequence then go to scan pattern against Swiss-Prot and TrEMBL. Paste in the Swiss-Prot and TrEmbl search page.

OR

To find proteins that have the potential phosphorylation sites, copy the optimum sequence then go to NCBI short peptide BLAST. Paste in the NCBI search text area.

OR

Make a query to Scansite. (recommended)

 

Step 2.

 

Paste a protein sequence with a possible phosphorylation site in the text area below. This method only works if there is a good match with the sequence motif. If this is the case, perform a Scansite search.

PASTE PROTEIN SEQUENCE IN THE TEXT AREA.

Step 3.

Step 4.

To find the structural location of the potential phosphorylation site, copy the unformatted target protein sequence and do a protein secondary structure prediction at any of the following prediction servers.

Pole Bio-Informatique Lyonnais

Predict Protein

3D PSSM

Others

Surface accessibility prediction (hydropathy)