Protein kinase	Predicted optimal heptapeptide substrate sequence	Optimal sequence based on oriented peptide library experiments	Natural Substrates
PKA	(RK)(RS)X(ST)(FLIM)(AVIS)(ILMKS)	(RH)(RKH)(RV)S(IFM)(IVF)(FIM)	(RK)(RK)(RK)(ST)(VLM)(SK)(PTSGN)
PKCa	(RK)(RKMST)X(ST)(FLIM)(RK)(FVILKRS)	(RK)(KQR)GS(FMIV)(KRF)(KFR)	(RKF)(RKTS)(LGSR)(ST)(LFV)(RKP)(RKSG)
PKCz	(RK)(RQM)X(ST)(FLIM)(AVI)(VFLK)	R(QKY)(GKM)S(FM)(FMA)(YK)	RQGSFF-
CaMKII	(RK)(QMLISTK)X(ST)(FLIM)(DES)(LIMKS)	(RQ)(QM)(QMK)S(FIML)(DEI)(LIMK)	(RK)(QRTIL)(AGS)(ST)(VLIMF)(AS)(GEQ)
PKCm	(RK)(QMTAS)X(ST)(VILMF)(AVI)(FLIMK)	R(QKEM)(MLK)S(VML)(AV)(FY)	RTASVAF
PHK	(RK)(QFMS)X(ST)(FLIM)(LIM)(LIM)	(RK)(MRQF)(MFL)S(FIM)(LI)(FLI)	(RK)(SQT)(GIL)(ST)(FI)(YRS)(EGS)
NIMA	(RF)RX(ST)(RR)(FLIM)	(FLM)(RK)(RK)S(RIVM)(RIMV)(MIFV)	FRSSIRR
CK2	(EDSQ)(EDHQ)X(ST)E(DERK)(SQNE)	(DE)(ED)(ED)S(EDA)(EA)(EA)	(DESP)(EDAV)(ES)(ST)(DES)(DE)(ED)
CDK2	(QH)(PMH)X(ST)P(RK)(RKHML)	H(PH)RSPRK
PKG	(RK)RX(ST)(FLI)(EQSN)(FLKST)	RKKSLAM	R(RK)(PR)(ST)(PR)(AS)(ETP)
PKCg	(RK)(RKM)X(ST)(FLIM)(RK)(FVLKR)	R(KRQ)(GK)S(FKM)(KR)(RK)
PKCd	(RK)(RKM)X(ST)(FLM)(RK)(FVLKR)	R(KRQ)(GA)S(FMV)(FKM)Y	MRQSVAV
AKT	(RK)(RKST)X(ST)(FLIM)(STNA)(VFLK)	RT(YFG)S(FM)(GTSRN)	R(TSR)(GST)(ST)(FIW)(PSA)(LRP)
Slk1 (Bck1)	(QNSA)(VPAR)X(ST)(LIM)(FLIA)(FLIK)	(RK)(FR)(GR)S(LIFM)(RIMF)(RFM)	(LA)(AV)()(ST)(FL)(TA)(TG)
Chk1	(RK)(FLA)X(ST)(LIM)(VILM)(FLIM)	R(YMP)(RF)S(FINM)(SA)(IFL)	RSPSMPE
Chk2	(RK)(ITS)X(ST)(FLID)(FL)(FLIK)	R(YKA)(YE)S(FI)(FIR)(YRF)	(RP)(STI)(FHKP)(ST)(DFM)(LVPS)(WLKE)
CKIg	(QNDA)(ED)X(ST)(LMI)(LI)(IFL)	E(TAG)GSI(IYF)(FY)	(SRN)(EST)(LES)(ST)(VIK)(ITY)(SREI)
CKId	(QNDA)(ED)X(ST)(LMI)(LI)F	E(TAG)GSI(IFY)(IGY)	(SRN)(EST)(LES)(ST)(VIK)(ITY)(SREI)
ERK1	(RKFL)(PVH)X(ST)P(PFLI)(RKHML)	(GPE)(PLI)(LMP)SP(GPF)(PFG)	(PVTL)(PV)L(ST)P(RSP)(PRKFG)
CDK5	(EQHS)(PKH)X(ST)P(RK)(RKHML)	H(HP)(KG)SP(KR)(HRK)	(EG)(TVH)(KA)(ST)P(VPE)K
bARK	(KRQEDN)(QMSTpY)X(ST)(DESN)(DESN)(KRQED)	-	(NTK)(NVT)(DpS)(ST)(ENDQ)(EDNG)(RQEDN)
S6K(1)	(RK)(RK)X(ST)(VLTIM)(QDNES)(LFKSA)	-	RRQSLIE
S6K(2)	R(IMT)X(ST)(ILM)(EDS)(LMK)	-	R(LTA)(SL)(ST)(LVH)(SR)(SA)
CaMKI	(RK)(QMITSK)X(ST)(VL.IM)(QSN)(KRQ)	-	RRLSDSN
AMPK	(RK)(RQMS)X(ST)V(AGVLF)(QNRKH)	-	(HRNS)(SRVN)(MAV)(ST)(FVEKG)(ALIS)(SNHDA)